col ii  (Servicebio Inc)

Journal: Journal of Nanobiotechnology

Article Title: Synergistic microenvironment therapy: a dual-function nanogel of supramolecular collagen ll-mimetic matrix and antioxidant MXene for attenuating intervertebral disc degeneration

doi: 10.1186/s12951-026-04211-w

Figure Lengend Snippet: The Composite Hydrogel Substrate Fosters a Pro-Anabolic and Anti-Catabolic Microenvironment for Adherent Nucleus Pulposus Cells. ( A ) Schematic of the in vitro culture model where NPCs are seeded on different hydrogel formulations. ( B ) Histochemical staining for matrix deposition by NPCs cultured on different hydrogels, including Alcian Blue (top row) and Toluidine Blue (bottom row) staining. Scale bar: 200 μm. ( C ) Western blot analysis of anabolic (COL-2, ACAN) and catabolic (MMP-13, ADAMTS-4) protein markers in NPCs cultured on different hydrogel substrates. GAPDH served as a loading control. ( D ) Dot plot visualization of the relative protein expression levels from Fig. 3E The size and color intensity of each dot correspond to the expression level. (E) Western blot analysis of key proteins in the integrin-mediated signaling pathway (p-FAK, p-ERK, p-p38). ( F ) Dot plot visualization of the relative protein expression levels from Fig. 3E. ( G ) Western blot analysis of anabolic and catabolic markers in the presence of an integrin α2β1 blocking antibody (iα2β1). ( H ) Dot plot visualization of the relative protein expression levels from Fig. 3G. ( I ) Representative immunofluorescence images for Collagen II (green), MMP-13 (red), and Aggrecan (green) in NPCs cultured on different hydrogels. Cell nuclei were counterstained with DAPI (blue). Scale bar: 100 μm. ( J ) Quantitative analysis of the relative fluorescence intensity for COL-2, MMP-13, and ACAN from the images in Fig. 3G. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant

Article Snippet: After treatment, cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum albumin for 1 h. Cells were then incubated overnight at 4 °C with primary antibodies against MMP-13, COL-II, ACAN, GPX4 and SLC7A11 (1:100; ProteinTech, Wuhan, China).

Techniques: In Vitro, Staining, Cell Culture, Western Blot, Control, Expressing, Blocking Assay, Immunofluorescence, Fluorescence

Journal: Journal of Nanobiotechnology

Article Title: Synergistic microenvironment therapy: a dual-function nanogel of supramolecular collagen ll-mimetic matrix and antioxidant MXene for attenuating intervertebral disc degeneration

doi: 10.1186/s12951-026-04211-w

Figure Lengend Snippet: The Mo 2 C@SAPH-R 16 GFOGER Hydrogel Attenuates IVDD Progression and Inhibits Ferroptosis In Vivo. ( A ) Photograph of the intra-discal injection procedure. ( B ) Schematic of the in vivo experimental design. ( C ) Representative X-ray images of rat tail intervertebral discs after 4 weeks and 8 weeks. ( D ) Representative T2-weighted MRI scans of the discs after 4 weeks and 8 weeks. ( E ) Quantitative analysis of the disc height index (DHI). ( F ) Quantitative analysis of the Pfirrmann grade. ( G ) Immunohistochemical staining for ACAN, COL-2, and ACSL4 in disc tissues at 8 weeks. Scale bar: 200 μm. ( H )Immunofluorescence staining for GPX4 in disc tissues at 8 weeks. Scale bar: 200 μm. ( I ) Quantitative analysis of glycosaminoglycan (GAG) content using DMMB assay. ( J ) Photograph of the biomechanical compression testing setup. ( K ) Quantitative analysis of the compressive modulus of the motion segments. Data are presented as mean ± SD( n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant

Article Snippet: After treatment, cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% bovine serum albumin for 1 h. Cells were then incubated overnight at 4 °C with primary antibodies against MMP-13, COL-II, ACAN, GPX4 and SLC7A11 (1:100; ProteinTech, Wuhan, China).

Techniques: In Vivo, Injection, Immunohistochemical staining, Staining, Immunofluorescence, Dimethylmethylene Blue Assay